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cell lines insect cell line sf9 expression systems n a hek 293 cells atcc  (Expression Systems Inc)


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    Expression Systems Inc cell lines insect cell line sf9 expression systems n a hek 293 cells atcc
    Cell Lines Insect Cell Line Sf9 Expression Systems N A Hek 293 Cells Atcc, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 99/100, based on 4762 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+insect+cell+line+sf9+expression+systems+n+a+hek+293+cells+atcc/Sf9+Cells/pm39977266-507-170-176
    Average 99 stars, based on 4762 article reviews
    cell lines insect cell line sf9 expression systems n a hek 293 cells atcc - by Bioz Stars, 2026-09
    99/100 stars

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    Recombinant:

    Article Title: Structural basis for ligand recognition of the human hydroxycarboxylic acid receptor HCAR3.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Flag antibody Thermo Fisher Scientific Cat# MA1-91878;RRID:AB_1957945 Alexa Fluor488-conjugated Beyotime Cat# A0428;RRID:AB_2893435 Chemicals, peptides, and recombinant proteins Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 Cholesterol hemisucinate (CHS) Anatrace Cat# CH210 Apyrase NEB Cat# M0398S Anti-Flag M1 antibody affinity resin Sigma Cat# A4596 Flag peptide Sigma Cat# F3290 3HO Bidepharm Cat# BD01001574 niacin MCE Cat# HY-B0143 compound 5c Wuxi AppTe N/A Lipofectamine 3000 reagents Invitrogen Cat# L3000001 IBMX Sigma Cat# I5879 forskolin Sigma Cat# F6886 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 Critical commercial assays Bac to Bac system Invitrogen Cat# A11098 cAMP-Gi kit Perkin Elmer Cat# TRF0263 GTPase-GLO assay Promega Cat# V7682 Deposited data 3HO-HCAR3-Gi complex EM Map This paper EMDB: EMD-36186 3HO-HCAR3-Gi complex coordinates This paper PDB: 8JEF niacin -HCAR3-Gi complex EM Map This paper EMDB: EMD-61502 niacin -HCAR3-Gi complex coordinates This paper PDB: 9JID compound 5c -HCAR3-Gi complex EM Map This paper EMDB: EMD-36189 compound 5c -HCAR3-Gi complex coordinates This paper PDB: 8JEI apo -HCAR3-Gi complex EM Map This paper EMDB: EMD-61501 apo -HCAR3-Gi complex coordinates This paper PDB: 9JIC Experimental models: Cell lines Insect cell line Sf9 Expression systems N/A HEK-293 cells ATCC Cat# CRL-1573 Recombinant DNA pFastBac-FLAG-HCAR3 This paper N/A pFastBac-Gai(G203A, A326S) This paper N/A pFastBac-Gb1g2 This paper N/A pFastBac-gp67-scFv16 This paper N/A pcDNA3.1-FLAG-HCAR3 This paper N/A pcDNA3.1-FLAG-HCAR2 This paper N/A Software and algorithms cryoSPARC Punjani et al., 201740 https://cryosparc.com/ Alphafold Zhuang et al., 202041 https://alphafold.ebi.ac.uk/ (Continued on next page) Cell Reports 43, 114895, November 26, 2024 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER COOT Emsley et al., 201042 https://www2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Phenix Adams et al., 201043 https://www.phenix-online.org/ UCSF Chimera Pettersen et al., 200444 https://www.cgl.ucsf.edu/chimera/ UCSF ChimeraX Goddard et al., 201845 https://www.cgl.ucsf.edu/chimerax/ PyMOL Schrodinger https://www.schrodinger.com/pymol Graphpad Prism GraphPad https://www.graphpad.com/ Modeller Webb et al. 201646 https://salilab.org/modeller/ Charmm-gui Jo et al. 200847 https://www.charmm-gui.org/ Gromacs Abraham et al.201548 https://www.gromacs.org/ MDAnalysis Open source https://www.mdanalysis.org/ Other 100 Holey Carbon film (Au, 300 mesh) QUANTIFOIL Cat# N1-C14nAu30-01 SuperoseTM 6 Increase 10/300 GL column GE Healthcare Cat# 29091596 Amicon Ultra Centrifugal Filters-100k Merck Cat# UFC9100


    Software:

    Article Title: Structural basis for ligand recognition of the human hydroxycarboxylic acid receptor HCAR3.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Flag antibody Thermo Fisher Scientific Cat# MA1-91878;RRID:AB_1957945 Alexa Fluor488-conjugated Beyotime Cat# A0428;RRID:AB_2893435 Chemicals, peptides, and recombinant proteins Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 Cholesterol hemisucinate (CHS) Anatrace Cat# CH210 Apyrase NEB Cat# M0398S Anti-Flag M1 antibody affinity resin Sigma Cat# A4596 Flag peptide Sigma Cat# F3290 3HO Bidepharm Cat# BD01001574 niacin MCE Cat# HY-B0143 compound 5c Wuxi AppTe N/A Lipofectamine 3000 reagents Invitrogen Cat# L3000001 IBMX Sigma Cat# I5879 forskolin Sigma Cat# F6886 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 Critical commercial assays Bac to Bac system Invitrogen Cat# A11098 cAMP-Gi kit Perkin Elmer Cat# TRF0263 GTPase-GLO assay Promega Cat# V7682 Deposited data 3HO-HCAR3-Gi complex EM Map This paper EMDB: EMD-36186 3HO-HCAR3-Gi complex coordinates This paper PDB: 8JEF niacin -HCAR3-Gi complex EM Map This paper EMDB: EMD-61502 niacin -HCAR3-Gi complex coordinates This paper PDB: 9JID compound 5c -HCAR3-Gi complex EM Map This paper EMDB: EMD-36189 compound 5c -HCAR3-Gi complex coordinates This paper PDB: 8JEI apo -HCAR3-Gi complex EM Map This paper EMDB: EMD-61501 apo -HCAR3-Gi complex coordinates This paper PDB: 9JIC Experimental models: Cell lines Insect cell line Sf9 Expression systems N/A HEK-293 cells ATCC Cat# CRL-1573 Recombinant DNA pFastBac-FLAG-HCAR3 This paper N/A pFastBac-Gai(G203A, A326S) This paper N/A pFastBac-Gb1g2 This paper N/A pFastBac-gp67-scFv16 This paper N/A pcDNA3.1-FLAG-HCAR3 This paper N/A pcDNA3.1-FLAG-HCAR2 This paper N/A Software and algorithms cryoSPARC Punjani et al., 201740 https://cryosparc.com/ Alphafold Zhuang et al., 202041 https://alphafold.ebi.ac.uk/ (Continued on next page) Cell Reports 43, 114895, November 26, 2024 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER COOT Emsley et al., 201042 https://www2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Phenix Adams et al., 201043 https://www.phenix-online.org/ UCSF Chimera Pettersen et al., 200444 https://www.cgl.ucsf.edu/chimera/ UCSF ChimeraX Goddard et al., 201845 https://www.cgl.ucsf.edu/chimerax/ PyMOL Schrodinger https://www.schrodinger.com/pymol Graphpad Prism GraphPad https://www.graphpad.com/ Modeller Webb et al. 201646 https://salilab.org/modeller/ Charmm-gui Jo et al. 200847 https://www.charmm-gui.org/ Gromacs Abraham et al.201548 https://www.gromacs.org/ MDAnalysis Open source https://www.mdanalysis.org/ Other 100 Holey Carbon film (Au, 300 mesh) QUANTIFOIL Cat# N1-C14nAu30-01 SuperoseTM 6 Increase 10/300 GL column GE Healthcare Cat# 29091596 Amicon Ultra Centrifugal Filters-100k Merck Cat# UFC9100

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